Partial Purification of α-amylase Expressed by a Tropical Mutant Strain of Aspergillus niger IFE 08; Comparative study of bacteriological analysis in hawked suya meat and suya meat on a Barbeque stand

E3 Journal of Microbiology Research

E3 Journal of Microbiology Research Vol. 1 (1) pp. 009-015, June 2013; © E3 Journals


Escherichia coli isolated from patients suspected for urinary tract infections in Hawassa Referral Hospital, Southern Ethiopia: An institution based cross sectional study

Mucheye Gizachew 1 * , Mulugeta Kebede2 , Yared Merid3 , Yenework Sinshaw4 , Martha Alemayehu1 , Martha Alemayehu1 , Fanaye Asfaw1 , Abate Assefa1 , Mulat Dagnaw1 , Agresew Alemu1
1 Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia
2 Department of Biology, Faculty of Natural and Computational Sciences, Hawassa University, Hawassa, Ethiopia
3 Department of Medical Microbiology, Immunology and Parasitology, College of Medicine and Health Sciences, Hawassa University, Hawassa, Ethiopia
4 Clinician in Gondar Teaching Hospital, ART Pediatric Clinic, University of Gondar, Gondar, Ethiopia
*Corresponding Author E-mail: muchegiza@gmail.com
Accepted 3 May 2013

Abstract

To investigate antimicrobial susceptibility and virulence patterns of uropathogenic E. coli isolated from patients visiting Hawassa Hospital, South Ethiopia. Cross-sectional study was conducted from December2011-March2012. Midstream urines were collected and streaked on MacConkey agar (Oxoid). Colony counts of 105 or more/ml of urine were regarded as significant bacteriuria. Identification was done using cultural characteristics and biochemical tests. Agar disc diffusion was used to test drug susceptibility pattern. Virulence tests were done using conventional methods. Data generated were analyzed using SPSS version 13. Ninety urine specimens were analyzed, 35.5% showed significant growth of E. coli. Ampicillin showed 100% drug resistance, Sulphamethoxazole/Trimethoprim (81.25%) and Ciprofloxacin and Chloramphenicol, both 43.8%. Multi-drug resistance showed in 81.25% isolates. Of those who had history of urinary tract infection, 77.77% exhibited resistance to three or more drugs. However, 96.87% susceptibility was seen to Nitrofurantion, Ceftriaxion (84.4%), Cefotaxime (81.25%) and Gentamicin (75.0%). Twenty eight percent isolates displayed hemolytic characters whereas 37.5% showed hydrophobicity. Many uropathogenic Escherichia coli showed haemolysin and cell surface hydrophobicity. Previous exposure to UTI was found to be the risk factor (p<0.05). Most uroisolates illustrated high level of drug resistance. So, clinicians shall base their decision on culture and drug susceptibility tests.

Keywords: Urinary tract infection;Antimicrobial susceptibility test;Virulence factor; Ethiopia

[Download Article - PDF]