E3 Journal of Biotechnology and Pharmaceutical Research
E3 Journal of Biotechnology and Pharmaceutical Research Vol. 2 (1) pp. 009-018 , October 2011; © E3 Journals; ISSN 2141-7474
Identification and validation of stable reference genes in Camellia SpeciesBornali Gohain * , Tirthankar Bandyopadhyay , Sangeeta Borchetia , Raju Bharalee , Sushmita Gupta , Priyadarshini Bhorali , Niraj Agarwala , Sudripta Das
Department of Biotechnology, Tea Research Association, Tocklai, Jorhat-785008, Assam, India
*Corresponding Author E-mail: email@example.com
Accepted 12 October 2011
We aimed at finding and validating a stable reference gene in Camellia sinensis and Camellia assamica from a set of four putative housekeeping genes in various samples exposed to different experimental conditions mainly biotic and abiotic stresses. Variation in gene expression across Camellia sinensis leaf tissues exposed to nine different kind of experimental sets was studied. The suitability of 18S rRNA, 26s rRNA, rubisco bis phosphatase (RuBP) and camellia actin (Act) as reference genes were validated by geNorm and BestKeeper programs and revealed 18S rRNA and RuBP to be the most stably expressing housekeeping gene. We therefore recommend use of RuBP as a stable reference gene for normalisation of transcripts abundance experiments in tea leaf samples.
Keywords: Gene expression; Housekeeping gene; Reference gene.[Download Article - PDF]