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E3 Journal of Scientific Research

E3 Journal of Scientific Research Vol. 4 (2) pp. 028-036, August 2016; © E3 Journals; ISSN 2276-9919
DOI: http://dx.doi.org/10.18685/EJSR(4)2_EJSR-16-016

Determination of the median lethal concentration of ΄Omo΄ and ΄Ariel΄ on Clarias gariepinus

Gambari Uthman Olatunji Bola1 *
1 Department of Science Laboratory, Delta State Polytechnic Ozoro, Nigeria
*Corresponding Author E-mail: u.gambari@gmail.com
Accepted 13 July 2016


The median lethal concentration of two detergents; “Omo” and “Ariel” on Clarias gariepinus (Catfish) were determined by comparing their behavioural responses after being exposed to these detergents at acute concentrations and to the control (without detergents).Juvenile of the African Catfish Clarias gariepinus mixed sex and the same broad stock mean weight and length (10.76± o.4g) and (10.50±0.32cm) respectively. Obtained from fishpond, and treated in vitro in large in large plastic aquaria of 50L capacity with well aerated borehole water. Acclimatized for 10 days, fed with commercial pellet food and later transferred to the experimental plastic aquaria. 10 fish per 20Laquarium. Concentrations of 0.05,0.07, 0.09, 0.1 and 0.15g/L detergents were prepared and introduced into the five plastic tanks in replicate of equal dimension and under the same environmental conditions. Ten (10) fingerlings of the fish were introduced in each tank while the control tank had no detergent. The behavior of the test 0rganisms were observed at 24, 48 72 and 96 hours after the treatment. It was found that the detergents are acutely toxic to the experimental fish due to the shooting up of the media΄s pH to the alkaline death point. It is therefore recommended that water bodies should be made clean to avoid contamination killing aquatic organisms

Keywords: Clarias gariepinus, Catfish, in vitro, lethal and acclimatized

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