Effect of Gentamicin and Amoxicillin on methicillin resistant Staphylococcus aureus (MRSA) against different time and concentrations; Comparative study of the effect of bitter leaf extract and antibiotics (gentamycin and amoxicillin) on bacterial species isolated from wound.

E3 Journal of Biotechnology and Pharmaceutical Research

E3 Journal of Biotechnology and Pharmaceutical Research Vol. 5 (2) pp. 18-23, May 2014; © E3 Journals; ISSN 2141-7474

In vitro propagation of Phaleria macrocarpa, God’s Crown

Nazirah Abdullah1 * , Nor Hasnida Hassan , Siti Suhaila A. Rahman , Haliza Ismail , Rozidah Khalid , Muhammad Fuad Yahya

1 Forestry Biotechnology Division, Forest Research Institute Malaysia (FRIM) 52109, Kepong, Selangor, Malaysia
*Corresponding Author E-mail: nazirah@frim.gov.my. Tel: 603-6279 7646 Fax: 603-62804614
Accepted 27 March 2014


Phaleria macrocarpa (Scheff.) Boerl, also known as God’s Crown, belongs to Thymelaeaceae family posses several medicinal properties such as anti-proliferative, anti-inflammatory and anti-angiogenic. In this paper, an attempt was made to develop a protocol for in vitro propagation of Phaleria macrocarpa. Seeds were used as explants for culture initiation and germinated in vitro after surface sterilization. Shoots multiplication in MS basal media supplemented with different concentrations of cytokinins were studied. BAP at 0.25 mg/l showed the most effective concentration for shoot multiplication with the highest number of shoots produced (4.27) and shoot elongation. For in vitro rooting, shoots were transferred to ½ MS basal media supplemented with auxins at different concentrations. The highest rooting frequency (83.33%) was observed in ½ MS basal medium. Regenerated plantlets were transferred to the nursery, 50% survivability was achieved and successfully acclimatized in mixed soil. The acclimatized plantlets were normal without any phenotypic aberration

Keywords: Phaleria macrocarpa, shoot multiplication, root induction and acclimatization

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