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E3 Journal of Biotechnology and Pharmaceutical Research

E3 Journal of Biotechnology and Pharmaceutical Research Vol. 5 (2) pp. 12-17, May 2014; © E3 Journals; ISSN 2141-7474

Exploration of different species of orange peels for mannanase production

Oladipo O. OLANIYI1 * , Charles A. OSUNLA2 , Olawale O. Olaleye3
1 Department of Microbiology, Federal University of Technology, P.M.B 704, Akure, Nigeria
2 Department of Microbiology, Adekunle Ajasin University, P.M.B 001, Akungba-Akoko, Ondo State, Nigeria
3 Nigerian Institute for Trypanosomiasis Research, P.M.B 2077, Kaduna, Nigeria
*Corresponding Author E-mail: microladit@gmail.com
Accepted 12 February 2014


Different species of orange peels were evaluated as carbon source in comparison to Locust Bean Gum for mannanase production by Penicillium italicum, Trichosporonoides oedocephalis and co-culture of P. italicum and T. oedocephalis. Mannanase activity was determined by dinitrosalicylic acid method. The protein concentration was however determined by the Lowry method. Of all the substrates screened, the highest mannanase activity (573.89 U/ml) was achieved with P. italicum cultured on sweet orange peels. The highest mannanase activity for mixed substrate fermentation in ratio 1:1:1:1 and 1:1:1:2 (Lime, Grape, Tangerine and Sweet orange peels) was obtained in a medium inoculated with P. italicum while mixed culture of P. italicum and T. oedocephalis gave maximum mannanase activity in mixed substrate ratio 2:1:1:1. The mixed substrate fermentation gave appreciable enzyme activity and thus, they can be used as low-cost substrate for mannanase production by these organisms

Keywords: Locust Bean Gum, mixed substrate fermentation, carbon sources

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