Farmers’ perceptions of climate change and its implication on livestock production in mixed-farming system areas of Bale highlands, Southeast Ethiopia; Challenges and extents of Soil and Water Conservation measures in Guba-Lafto Woreda of North Wollo, Ethiopia; Evaluation of milk production performance of lactating Fogera cows fed with urea and effective micro-organisms treated rice straw as basal diet.

E3 Journal of Agricultural Research and Development

E3 Journal of Agricultural Research and Development Vol. 4 (4) pp. 054-065, April 2014; © E3 Journals; ISSN 2276-9897

Screening and the effect of extracts of Thevetia peruviana on the development of Colletotrichum gloeosporioides, causal agent of cassava anthracnose disease

Jules Patrice Ngoh Dooh 1 * , Zachee Ambang 1 , Anasthasia Tih Ewola2 , Alain Heu 1 , Philippe Kosma3 , Edson Josian Maho Yalen4 , Raphael Tih Goghomu2
1 Laboratory of Plant Pathology, Department of Plant Biology, Faculty of Science, University of Yaoundé I (Cameroon). BP 812 yde
2 Department of Organic Chemistry, Laboratory of recovery of plant biomass, University of Yaoundé I (Cameroon)
3 The Higher Institute of Sahel, University of Maroua (Cameroon), BP 46 maroua
4 The Teacher Higher School of University of Yaounde I, BP 812 Yde
*Corresponding Author E-mail: ndjuliopat@yahoo.fr
Accepted 29 March 2014

Abstract

The objective of this work was to carry out the screening of extracts from seeds of Thevetia peruviana (Pers.) K. Schum. and assess the effect of the extracts on the in vitro and in vivo development of Colletotrichum gloeosporioides that causes anthracnose of cassava. Five extracts obtained by soxhlet apparatus (extracts of hexane, ethyl acetate, acetone, methanol and the aqueous extract) were used. One fungicide (mancoxyl) served as control reference. Five strains (DB 1.1, 5a local, ES 4.1, Lit po and Bam 1.1) from five regions of Cameroon were tested. Three extracts concentrations (6.25, 12.5, and 25 µl/ml) and one concentration of fungicide (62 µg/ml) were used in PDA medium. The screening performed revealed the presence of several chemical families such as, coumarins, phenols, alkaloids, sugars, terpenes, sterols and flavonoids. The acetone, methanol and aqueous extracts inhibited the growth of strains with inhibition percentage ranging from 11.90 %-93 %. Only acetone extract proved active in the inhibition of spore germination of C. gloeosporioides with a percentage of inhibition of nearly 100 % at all concentrations. The MIC50 and MIC90 (minimum inhibitory concentrations) were determined. The in vivo test carried out on detached leaves has confirmed the effectiveness of these extracts in reducing the severity of the disease. These extracts could be used in the integrated control of the plant pathogens tested.

Keywords: Colletotrichum gloeosporioides; Thevetia peruviana; screening; extracts; inhibition.

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