E3 Journal of Biotechnology and Pharmaceutical Research
E3 Journal of Biotechnology and Pharmaceutical Research Vol. 4 (1) pp. 1-7, January 2013; © E3 Journals; ISSN 2141-7474
Cyclo (L-phenyl, L-prolyl). Diketopiperazines from a newly isolated Streptomyces sudanensis. A4.4
Abdelhalem A. Hamza1 * , Hiba A. Ali1 , Benjamin R. Clark2 , Cormac D. Murphy2 , Elsheik A. Elobied31 National Centre for Research, Commission for Biotechnology and Genetic Engineering, Khartoum, Sudan
2 School of Biomolecular and Biomedical Science, Centre for Synthesis and Chemical Biology, Ardmore House, University College Dublin, Dublin 4, Ireland
3 Ahfad University for women, College of Pharmacology, Khartoum, Sudan
*Corresponding Author E-mail: aahamzaa@yahoo.com, Tel: +249907331399, Fax: 00249-183-770701.
Accepted 9 January 2013
Abstract
A new actinomycete strain designated A4.4 possessing strong antibiotic properties was isolated from soil samples collected from different geographical areas in Sudan. The isolate was taxonomically characterized on the basis of morphological, physiological and phylogenetic analyses, which have properties similar to Streptomyces strain. In order to confirm the identified isolate, 16S rRNA gene (1.277 k p) analysis of the isolate was studied. The comparison of the 16S rRNA sequence of the A 4.4 isolate with those sequences submitted to GenBank demonstrated that the strain was 94% similar to the 16S rRNA sequence to number of isolates all of which were Streptomyces species, but phylogenetic analysis demonstrated that the Sudanese strain was on a different node to previously identified strains. Also it was readily distinguished strain A4.4 from its closest phylogenetic neighbors of these species by using a combination of phenotypic properties; these species are distinct from the known producers of Cyclo (L-phenyl, L-prolyl). On the basis of these results, strain A4.4 is proposed as the type strain of the novel species, for which the name Streptomyces sudanensis. A4.4 is proposed. The bioactive compound was isolated from the strain and was revealed to be cyclo (L phenyl, L-prolyl) by UV, 1H- and 13C-NMR and MS analyses and by comparison with reference data from literature
Keywords: Streptomyces sudanensis, A4.4, cyclo(L-phenyl, L-prolyl), 16S rRNA
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