Effect of Gentamicin and Amoxicillin on methicillin resistant Staphylococcus aureus (MRSA) against different time and concentrations; Comparative study of the effect of bitter leaf extract and antibiotics (gentamycin and amoxicillin) on bacterial species isolated from wound.

E3 Journal of Biotechnology and Pharmaceutical Research

E3 Journal of Biotechnology and Pharmaceutical Research Vol. 4 (1) pp. 8-13, January 2013; © E3 Journals; ISSN 2141-7474

Antibacterial activity of Nigella sativa seed in various germination phases on clinical bacterial strains isolated from human patients

Mohammad Hayatul Islam1 * , Iffat Zareen Ahmad1 , Mohammad Tariq Salman2

1 Department of Bioengineering, Integral University, Dasauli, Kursi Road, Lucknow-226026, Uttar Pradesh, India
2 Department of Pharmacology, Era’s Lucknow Medical College, Lucknow, Uttar Pradesh, India
*Corresponding Author E-mail: Email: iffat77@rediffmail.com, iffat77@gmail.com. Tel: +919919273517. Fax: +91-522-2890809
Accepted 23 December 2012


Nigella sativa is an important spice and flavoring agent which is widely used in various European and Asian cuisines. It harbors an array of medicinal properties as shown by various researches. Germination is a phenomenon during which rapid changes in metabolic activities and the interconversions of metabolites take place. The objective of present study was to evaluate the antibacterial activity of N. sativa seed that are on various germination phases against clinical bacterial strains isolated from pus, urine, ascitic fluid and cerebrospinal fluid of various patients. The minimum inhibitory concentration (MIC) values were determined by using a modified macro-broth dilution technique. The agar well diffusion method was used to test the antimicrobial effects of N. sativa extracts. Some broad spectrum antibiotics were used as positive control. The phytochemical constituents of N. sativa seed were also studied in germination phases. The distilled methanolic extracts of N. sativa showed significant antimicrobial activity against tested clinical strains of Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus mirabilis bacteria. Results showed day-dependent and dose-dependent activity and a significant antimicrobial effect was observed as germination proceeded

Keywords: Nigella sativa seed, germination phases, phytochemicals, clinical isolates, antibacterial activity

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