E3 Journal of Environmental Research and Management
E3 Journal of Environmental Research and Management Vol. 4 (1) pp. 0163-0170, January 2013; © E3 Journals; ISSN 2141-7466
Effect of salinity on growth, chlorophyll, carbohydrate and protein contents of transgenic Nicotiana Plumbaginifolia over expressing P5CS geneRiahy Mahboobeh 1 , Ehsanpour Ali Akbar 2 *
1 Department of Biology, University of Isfahan, Isfahan, Iran
*Corresponding Author E-mail: email@example.com
Accepted 13 January 2013
Transgenic tobacco plant (Nicotiana Plumbaginifolia) over expressing P5CS (Î”-pyrroline-5-carboxylate synthetase) and non transgenic plant as control were grown on MS medium supplemented with 0, 100, 150, 200 and 250 mM NaCl for 28 days. The effect of NaCl concentrations on fresh and dry weight, the chlorophyll (chl. a and b and total), carbohydrate and protein content were measured. Results showed a significant increase of chlorophyll, fresh weight, dry weight and carbohydrate in transgenic compared to the non transgenic plants under salt treatments. The total protein was decreased with increasing of salt concentration.
Keywords: Salt stress, P5CS, Nicotiana plumbaginifolia, Proline[Download Article - PDF]