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E3 Journal of Environmental Research and Management

E3 Journal of Environmental Research and Management Vol. 4 (1) pp. 0163-0170, January 2013; © E3 Journals; ISSN 2141-7466

Effect of salinity on growth, chlorophyll, carbohydrate and protein contents of transgenic Nicotiana Plumbaginifolia over expressing P5CS gene

Riahy Mahboobeh 1 , Ehsanpour Ali Akbar 2 *
1 Department of Biology, University of Isfahan, Isfahan, Iran
*Corresponding Author E-mail: ehsanpou@sci.ui.ac.ir
Accepted 13 January 2013

Abstract

Transgenic tobacco plant (Nicotiana Plumbaginifolia) over expressing P5CS (Δ-pyrroline-5-carboxylate synthetase) and non transgenic plant as control were grown on MS medium supplemented with 0, 100, 150, 200 and 250 mM NaCl for 28 days. The effect of NaCl concentrations on fresh and dry weight, the chlorophyll (chl. a and b and total), carbohydrate and protein content were measured. Results showed a significant increase of chlorophyll, fresh weight, dry weight and carbohydrate in transgenic compared to the non transgenic plants under salt treatments. The total protein was decreased with increasing of salt concentration.

Keywords: Salt stress, P5CS, Nicotiana plumbaginifolia, Proline

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