E3 Journal of Medical Research
E3 Journal of Medical Research Vol. 1 (7) pp. 091-094, August 2012; © E3 Journals; ISSN 2276-9900
Production of amylase from Aspergillus niger using a defined synthetic growth medium and also rice (Oryza sativa) as growth substrate
Adekunle Odunayo Adejuwon1 * , Olanike Olajide2 , Olabisi Adewole21 Department of Microbiology,Faculty of Information Technology and Applied Sciences,Lead City University,Ibadan,Nigeria
2 Nike Ventures,Yawiri, Akobo-Ojurin,Ibadan,Nigeria
*Corresponding Author E-mail: adejuwon_ao@yahoo.com
Accepted 6 June 2012
Abstract
Aspergillus niger grew in both a growth medium with rice as carbon and growth source and in a defined synthetic medium with varying carbon and nitrogen sources at 25oC producing amylase. Optimum amylase activity in rice was expressed on the eighth day of incubation as 0.58 Units. In the synthetic growth medium with starch as carbon source and tryptone as nitrogen source, optimum amylase activity was expressed on the seventh day as 0.47 Units; with ammonium chloride as nitrogen source and maltose as carbon source of growth, optimum amylase activity was expressed on the ninth day as 3.525 Units. This investigation suggests a means of production of amylase for industrial purposes.
Keywords: Aspergillus niger; Amylase; synthetic growth medium; rice
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