E3 Journal of Medical Research
E3 Journal of Medical Research Vol. 6 (3) pp. 022-026, May 2017; © E3 Journals; ISSN 2276-9900
DOI: http://dx.doi.org/10.18685/EJMR(6)3_EJMR-17-013
Antioxidant activity and in-vitro Potential inhibition of Nigella sativa and Saussurea lappa against LDL oxidation among Sundance
Isam Sadik1 * , Alawiya Abd Allah2 , Mohammed Abdulhameed3 , Mashair Abdelkader4 , Nazra Sayed5 , Anhar Nour6 , Abid Algadir Tom71 Department of Clinical Chemistry, Faculty of Laboratory Science, Omdurman Islamic University
2 Department of Clinical Chemistry, Faculty of Laboratory Science, Omdurman Islamic University
3 Department of Pathology, Faculty of Medicine, Omdurman Islamic University
4 Department of Pediatric, Faculty of Medicine, Neelain University
5 Ministry of Health, Turkey
6 Department of Clinical Chemistry, Faculty of Laboratory Science, Omdurman Islamic University
7 Department of Clinical Chemistry ,University of Tigana
*Corresponding Author E-mail: isnaalah@yahoo.com
Accepted 23 April 2017
Abstract
ItÍ´s well documented that oxidized low density lipoprotein (LDL) plays a major role in the modification of atherosclerosis. Nigella sativa (NS) and Saussurea lappa (SL) in literature documented as a good antioxidant.To undertaken to evaluate the ability of NS and SL for inhibition of LDL oxidation in-vitro, in fact Nigella sativa and Saussurea lappa LDL was collected by ultracentrifugation and enhanced to oxidize by CuSO4 and NS and SL were used to inhibit LDL oxidation in-vitro. Free radical scavenging of 50% of radicals of DPPH and phenolic content of both herbals were measured to assess the antioxidant activity and compared with standard antioxidant (vitamin E). The inhibitory effect of NS, SL and vitamin E against oxidation ratio was significantly better than in non addition of both herbals (p<0.01); NS was better than SL (p<0.01); and comparable with vitamin E. Both herbals possess good antioxidant activity against DPPH radicals and have high phenolic contents enable it to inhibit LDL oxidation in-vitro. NS and SL possess strong antioxidant activity comparable with vitamin E and good for cardiovascular health.
Keywords: Nigella sativa, Saussurea lappa, vitamin E, LDL oxidation, antioxidant activity.
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