E3 Journal of Microbiology Research
E3 Journal of Microbiology Research Vol. 3 (1) pp. 001-004, January 2017; © E3 Journals
DOI: http://dx.doi.org/10.18685/EJMBR(3)1_EJSR-16-024
Partial Purification of α-amylase Expressed by a Tropical Mutant Strain of Aspergillus niger IFE 08
Adejuwon AO1 * , Adedayo O2 , Olutiola PO3 , Segal SJ41 Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria
2 Department of Microbiology, Babcock University, Ilishan Remo, Ogun State, Nigeria
3 Department of Biological Sciences, Bowen University, Iwo, Nigeria
4 Director, Division of Population Sciences, The Rockefeller Foundation, Manhattan, New York, United States of America/Adjunct Professor of Clinical Pharmacology, Weill Medical College, Cornell University, Manhattan, New York, United States of America
*Corresponding Author E-mail: ao_adejuwon@yahoo.ca
Accepted 3 October 2016
Abstract
A mutant of Aspergillus niger induced by ultraviolet radiation of a strain of a tropical wild type (Aspergillus niger IFE 08) expressed α-amylase activity in a defined medium with starch as carbon source and ammonium chloride as nitrogen source of growth and development. The enzyme was subjected to partial purification by ammonium sulphate precipitation followed by dialysis. Specific activity of the enzyme increased 1.71 fold while recovery was 29.9% after dialysis.
Keywords: Aspergillus niger, tropical, mutant strain, partial purification, α-amylase
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