E3 Journal of Biotechnology and Pharmaceutical Research
E3 Journal of Biotechnology and Pharmaceutical Research Vol. 5 (2) pp. 18-23, May 2014; © E3 Journals; ISSN 2141-7474
In vitro propagation of Phaleria macrocarpa, God’s Crown
Nazirah Abdullah1 * , Nor Hasnida Hassan , Siti Suhaila A. Rahman , Haliza Ismail , Rozidah Khalid , Muhammad Fuad Yahya1 Forestry Biotechnology Division, Forest Research Institute Malaysia (FRIM) 52109, Kepong, Selangor, Malaysia
*Corresponding Author E-mail: nazirah@frim.gov.my. Tel: 603-6279 7646 Fax: 603-62804614
Accepted 27 March 2014
Abstract
Phaleria macrocarpa (Scheff.) Boerl, also known as God’s Crown, belongs to Thymelaeaceae family posses several medicinal properties such as anti-proliferative, anti-inflammatory and anti-angiogenic. In this paper, an attempt was made to develop a protocol for in vitro propagation of Phaleria macrocarpa. Seeds were used as explants for culture initiation and germinated in vitro after surface sterilization. Shoots multiplication in MS basal media supplemented with different concentrations of cytokinins were studied. BAP at 0.25 mg/l showed the most effective concentration for shoot multiplication with the highest number of shoots produced (4.27) and shoot elongation. For in vitro rooting, shoots were transferred to ½ MS basal media supplemented with auxins at different concentrations. The highest rooting frequency (83.33%) was observed in ½ MS basal medium. Regenerated plantlets were transferred to the nursery, 50% survivability was achieved and successfully acclimatized in mixed soil. The acclimatized plantlets were normal without any phenotypic aberration
Keywords: Phaleria macrocarpa, shoot multiplication, root induction and acclimatization
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