E3 Journal of Biotechnology and Pharmaceutical Research

E3 Journal of Biotechnology and Pharmaceutical Research Vol. 4 (7) pp. 110-116, July 2013; © E3 Journals; ISSN 2141-7474

Optimization studies on mannanase production by Trichosporonoides oedocephalis in submerged state fermentation

Abstract

The study evaluated various agricultural wastes and process parameters for mannanase production by Trichosporonoides oedocephalis in submerged state fermentation. Different agricultural wastes were screened as substitutes to commercial substrate for mannanase production using substitution method. Process parameters affecting enzyme production were optimized using one-factor-at-a-time approach. Mannanase production was conducted in mineral salt medium and enzyme activity determined by dinitrosalicylic acid method. Among tested carbon sources, cassava peels proved to be the best for mannanase production (11.767 U/ml), while the lowest (0.100 U/ml) was observed with pineapple peels. Maximum mannanase activity and productivity (37.963 U/ml, 4.745 U/ml/hr) were observed after 96 hrs of incubation. Initial pH of the culture medium was also optimized and it was observed that enzyme activity increased as the pH of fermentation medium increased. The best nitrogen sources and inoculum concentrations (spores/ml) were ammonium nitrate and 1×106, respectively. The optimal culture conditions obtained from this study helped to standardize the requirements for optimum production of mannanase using agrowastes, thereby contributing to better fish feed formulation incorporating plant ingredients, especially in the larval stages of fish fingerlings when the enzyme system is not efficient

Keywords: Trichosporonoides oedocephalis, mannanase, agro-wastes, submerged fermentation, optimization