E3 Journal of Biotechnology and Pharmaceutical Research

E3 Journal of Biotechnology and Pharmaceutical Research Vol. 4 (4) pp. 54-60, April 2013; © E3 Journals; ISSN 2141-7474

Phytochemical screening and evaluation of in-vitro antioxidant potential of Monotheca buxifolia

Abstract

The present study aimed to investigate the antioxidant potential of Monotheca buxifolia leaves (ML) by employing some in vitro methods. The antioxidant components were initially extracted from the plant in methanol and were further fractionated in solvents of different polarity. The Phytochemical screening showed that the leaves of Monotheca buxifolia are naturally enriched with anthraquinones, flavanoids, reducing sugars, terpenoids, tannins, saponins, and cardiac glycosides. The trolox equivalent antioxidant capacity (TEAC) and Ferric reducing antioxidant power values for leave extracts were ranged from 30.3-109.2 mM of Trolox equivalents and 8.9-29.9 mg/L of FeSO4 equivalents respectively. The EC50 values of leaves fractions were found to be in range 14.4-172.8 (mg of dried mass/mL). Using Total phenolic contents assay, the amount of total phenolics varies from 39.2-131.8 mg/L of gallic acid equivalents for leaves. The total lavanoids content were found to be 262.75-825.45 mg/L of Quercetin for leaves and fruit fractions. The percentage bound iron for metal chelating activity varies from 69-73%. The percentage scavenging activity of superoxide anions radical was 61-73% for leave extracts. On the basis of these results obtained here ML may be considered as a rich source of antioxidant

Keywords: Antioxidant activity, Monotheca buxifolia, phytochemical screening, TEAC value, EC50