E3 Journal of Biotechnology and Pharmaceutical Research
E3 Journal of Biotechnology and Pharmaceutical Research Vol. 4 (3) pp. 48-53, March 2013; © E3 Journals; ISSN 2141-7474
Evaluation of hypoglycemic, antihyperglycemic and antidiabetics properties of Trilepisium madagascariense D.C. Leeuwenberg (Moraceae)
Raoul AMPA1 * , Gabriel AHOMBO1 , Etienne NGUIMBI1 , Martin DIATEWA2 , Théophile DIMO3 , Jean Maurille OUAMBA1 , Ange Antoine ABENA21 Laboratoire de Biologie Cellulaire et Moléculaire, Faculté.des Sciences et Techniques, Université Marien NGOUABI, République du Congo
2 Laboratoire de Biochimie et de Pharmacologie, Faculté des Sciences de la santé, Université Marien NGOUABI, République du Congo
3 Laboratoire de Biologie et Physiologie Animales, Faculté des Sciences, Université de Yaoundé 1, République du Cameroun
*Corresponding Author E-mail: ampa_ampa@yahoo.fr.
Accepted 20 March 2013
Abstract
Study was carried out to investigate the anti-diabetics activity of Trilepisium madagascariense leaves used in traditional medicine to heal diabetes. Experiments were conducted on normoglycemic rats for hypoglycemia activity, on rats that were made hyperglycemic by orally loading (3 g/Kg) of glucose at 10% for anti-hyperglycemia activities and on rats made diabetics by injection of streptozotocin for the anti-diabetics activities. The aqueous decoction (400 and 800 mg/Kg, per os) and the macereted hydro-ethanolic of the same doses, per os produces a hypoglycemia from the second hour up the sixth hour when this activity became more significant (p<0.001) (n=5). All extract administered an hour ahead, the glucose overcharged has show a protective effect since they become, as well as glibenclamide, hyperglycemia which come upon half an hour after overloading the glucose, comparatively to control rats which have received distilled water with p<0.001 (n=5). From diabetics rats, in the two type of extracts, the total or row flavonoïdes, as well as insulatard (5 UI/Kg) decreases significantly the glycemia one hour ahead and the activity is maintained up to the 5th hour (p<0.001). These results suggest that the Trilepisium madagascariense leaves contains anti-diabetic compound and justify their traditional use in diabetes cure.
Keywords: Diabetes, hypoglycemic, anti-hyperglycemic, antidiabetics, Trilepisium madagascariense
[Download Article - PDF]
