E3 Journal of Biotechnology and Pharmaceutical Research
E3 Journal of Biotechnology and Pharmaceutical Research Vol. 4 (3) pp. 42-47, March 2013; © E3 Journals; ISSN 2141-7474
The effect of glutathione, catalase and ß-carotene on free radicals using model diseases as reference
Ananya Sen1 , Vinaya B. Joseph1 , Kavitha G. Singh2 *1 Department of Life Science, Post Graduate Studies, Mount Carmel College, No. 58, Palace Road, Bangalore, India- 560052
2 Department of Chemistry, Mount Carmel College, No. 58, Palace Road, Bangalore, India- 560052
*Corresponding Author E-mail: kavi182@yahoo.co.in. Tel: +91-80-22261759
Accepted 4 March 2013
Abstract
The study was designed to find out the correlation between lipid peroxide (LPO) levels and the severity and complication of stress related disorders with a focus on the potential benefit of antioxidants in the treatment of chronic diseases, as antioxidants play an important role in maintaining a balance between free radicals produced by metabolism or derived from environmental sources. The antioxidants used were glutathione, catalase enzyme and ß- carotene, all of which have been implicated in antioxidant reactions that occur in the body. The lipid peroxidation was assessed by measuring the concentration of plasma malondialdehyde (MDA). The TBA test was performed to measure the amount of MDA present in the sample. The MDA-TBA complex was measured by visible absorption spectrophotometry. The ability of these antioxidants to scavenge 1, 1-diphenyl-2-picrylhydrazyl (DPPH) was also measured by spectrophotometry. In the following study, it was seen how the antioxidants have the ability to slow, if not stop, the harmful degenerative effect of free radicals in oxidative stress related disorders by decreasing the lipid peroxide levels.
Keywords: Lipid peroxides, MDA, DPPH, Antioxidants, Glutathione, Catalase, β-carotene
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