E3 Journal of Biotechnology and Pharmaceutical Research
E3 Journal of Biotechnology and Pharmaceutical Research Vol. 4 (2) pp. 22-28, February 2013; © E3 Journals; ISSN 2141-7474
Acute and subacute toxicity studies of "Hémodya", aqueous extract of three medicinal plants, used to fight against sickle cells disease in albino rats
Kotué, T. C1 * , Ndomou, M2 , Pieme, A. C1 , Oumdoum, P.C3 , Ngogang, Y. J11 Laboratoire de Biochimie, de Physiologie et de Pharmacologie de la Faculté de Médecine et des Sciences Biomédicales/CHU, Université de Yaoundé 1, BP. 482 Yaoundé, Cameroun
2 Laboratoire de Biochimie de la Faculté des Sciences, Université de Douala, BP. 24157 Douala, Cameroun
3 Laboratoire de Biologie animale de la Faculté des Sciences, Université de Douala, BP. 24157 Douala, Cameroun
*Corresponding Author E-mail: ctkotue_bio@yahoo.fr. Tel: 237 77 22 34 18.
Accepted 27 January 2013
Abstract
The present study was carried out to evaluate the acute and subacute toxicity of “Hémodya†a phytochemical drug used to fight against sickle cells disease. This drug is a combination of aqueous extract of three medicinal plants: Cassia siamea Lam (Euphorbiacea); Delonix regia, le flamboyant (Caesalpiniaceae) and Garcinia cowa Rox (Guttiferae) via the oral route in albino rats. For the acute toxicity test, a single dose administration of 5 g/kg bw. of “Hémodya†did not produce any toxic signs or deaths in rats. There were no signiï¬cant differences (p>0.05) in the body and organ weights between control and treated groups. The (LD50) of “Hémodya†was higher than 5 g/kg bw. In subacute toxicity study, no mortality and toxic signs were observed with the doses of 90, 180, 360 or 720 mg/kg bw. of "Hémodya" for 28 consecutive days. There were no significant differences in the body and organ weights between the control and treated animal of both sexes. Hematological analysis showed a significant difference (p<0.05) between hemoglobin and hematocrit estimations of the control and treatment groups with "Hémodya" after 28 days. However, no changes were observed in biochemical parameters of both sexes. Pathologically, neither gross abnormalities nor histopathological changes were observed. These results suggest that "Hémodya" did not produce toxic effects in albino rats. Collectively, these data demonstrate that it has a high margin of traditional drug safety.
Keywords: Key words: Sickle cells disease, "Hémodya", medicinal plants, acute, subacute toxicity.
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