E3 Journal of Biotechnology and Pharmaceutical Research

E3 Journal of Biotechnology and Pharmaceutical Research Vol. 3 (9) pp. 141-148, December 2012; © E3 Journals; ISSN 2141-7474

Antibacterial and antifungal activity of different extracts of Datura stramonium (branches and leaves sample)

Abstract

Datura stramonium branches and leaves sample in three different solvents benzene, chloroform and ethanol was extracted and assessed for antibacterial and antifungal activity. The antibacterial activity was checked against Enterobacter (clinical strain/PIMS), Micrococcus luteus (clinical strain/PIMS), Pseudomonas aeruginosa (clinical strain/PIMS), E.coli ATCC 25922, Staphylococcus aureus (clinical strain/PIMS) and Klebsiella pneumonia ATCC 700603. Datura stramonium chloroform extract produced maximum zone of inhibition 16±0.7mm against Enterobacter while minimum of 7±0.7mm against K. pneumonia. Benzene extract of the plant exhibited maximum of 15±0.7mm of zone of inhibition against Enterobacter and M. luteus while minimum of 9±0.3mm against S. aureus and K. pneumonia and ethanol extract of D. stramonium gave maximum zone of inhibition against K. pneumonia while minimum against E.coli. The MBC values revealed that benzene extract (3.12mg/ml) was effective against P. aeruginosa while same concentration of chloroform extract was very active against S. aureus, P. aeruginosa and M. luteus.All the extracts of D. stramonium have shown significant antifungal activity against Saccharomyces cerevisiae, Aspergillus fumigatus and Aspergillus niger with maximum antifungal activity against S. cerevisiae and zone of inhibition was about 16±0.2mm by ethanol extract, 15±0.3mm by chloroform and 14±1.6mm by benzene extract while minimum antifungal activity was observed against A. niger.

Keywords: Datura stramonium extract, Zone of inhibition, Antibacterial activity, Antifungal activity, Agar well diffusion method, Minimum inhibitory concentration