E3 Journal of Biotechnology and Pharmaceutical Research
E3 Journal of Biotechnology and Pharmaceutical Research Vol. 3 (8) pp. 137-140, October 2012; © E3 Journals; ISSN 2141-7474
In vitro evaluation of the effects of acetone, on the potency of cisplatin: Is it a good candidate for cisplatin carrier preparation?
Hasan Ebrahimi Shahmabadi1 , Azim Akbarzadeh1 * , Mohammad Javad Mokhtari2 , Mehri Mortazavi1 , Soheil Ghasemi1 , Hadi Mohammadi3 , Seyed Kazem Bagherpour Doun41 Pilot Biotechnology department, Pasteur Institute of Iran, Tehran, Iran
2 Young Researches Club, Zarghan Branch, Islamic Azad University, Zarghan, Iran
3 Young Researches Club, Kermanshah Branch, Kermanshah, Iran
4 Department of biochemistry and Biophysics, Babol University of Medical Sciences, Babol, Iran
*Corresponding Author E-mail: azimakbarzadeh@yahoo.com, Tel: +9821-6646 5406, Fax: +9821-6646 5132
Accepted 7 August 2012
Abstract
One of the major problems for cisplatin nanocarrier production has originated in the low water solubility of this anticancer drug. The solubility power of acetone as an organic solvent and its effect on antitumor activity of cisplatin in order to produce a drug carrier constituents was therefore evaluate. Powder of cisplatin (secondary cisplatin) was obtained after evaporation of acetone. Its potency was determined by MTT and MIC tests using Hep-G2 cell line and E. coli respectively. A further insight into the similarity between two chemical compounds has also performed using Fourier Transform Infrared Spectroscopy technique. Compared with control groups, there was no significant difference between primary and secondary cisplatin cytotoxicity by MTT assay. MIC results also did not show significant discrepancy (MIC for primary and secondary cisplatin were 9.5 and 9.7 micro molar respectively). The similar spectra obtained of primary and secondary cisplatin at FTIR indicated these compound were approximately identical. Based on the results of current study it can be concluded that under proper condition, acetone as an organic solvent can be considered for cisplatin carrier preparation.
Keywords: Cisplatin, Acetone, Hep-G2 cell line, Drug delivery.
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