Effect of Gentamicin and Amoxicillin on methicillin resistant Staphylococcus aureus (MRSA) against different time and concentrations; Comparative study of the effect of bitter leaf extract and antibiotics (gentamycin and amoxicillin) on bacterial species isolated from wound.

E3 Journal of Biotechnology and Pharmaceutical Research

E3 Journal of Biotechnology and Pharmaceutical Research Vol. 3 (5) pp. 88-93, July 2012; © E3 Journals; ISSN 2141-7474


In vitro shoot induction of Acacia auriculiformis from juvenile and mature sources

Haliza Ismail1 * , Noraini, Abdul Shukor2 , Mohd Yusoff3 , Nor Hasnida Hassan1 , Fadhilah Zainudin1 , Nazirah Abdullah1 , Siti Suhaila Abdul Rahman1
1 Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia
2 Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia
3 University Selangor, Bestari Jaya, 45600, Selangor Darul Ehsan, Malaysia
*Corresponding Author E-mail: haliza@frim.gov.my, Tel: +603-62797155, Fax: +603-62804614
Accepted 25 May 2012

Abstract

Nodal segments of Acacia auriculiformis from juvenile (5 month-old and 14 month-old) and mature (72 month- old) genotypes were micropropagated through regular regime for more than 36 months in vitro. Shoot induction utilized six concentrations of BAP for 5 month-old and 72 month-old sources while 14 month-old and 72 month-old sources were treated with a combination of similar concentrations of BAP and 0.1 mg/l and 0.5 mg/l Kn respectively in Murashige and Skoog medium supplemented with 3 % sucrose and 3.75 g/L Gelrite. Nodal segments from both the juvenile and mature sources can be sustainably micropropagated in tissue culture. Overall, the juvenile materials displayed higher potential for axillary shoot induction and growth than the explants from mature sources for both treatments. Age of stock plant (juvenility of the explant) plays an important role on the success of tissue culture initiation. The highest shoot regeneration (100%) was obtained from the explants of 5 month-old seedlings whereas 85.71% of shoot regeneration was from explants of 72 month-old tree. For the combination of BAP with Kinetin, the highest percentage of shoot regeneration of 14 month-old seedling was 66.67 % with mean length of shoots 6.80 mm were obtained from explants cultured in 0.5 mg/L BAP plus 0.1 mg/L Kn whereas for nodal segments of 72 month-old tree, the highest percentage of shoot regeneration was 50% and mean length of shoots was 2.00 mm were in 0.1 mg/L BAP plus 0.1 mg/L Kn. It was observed that higher percentages of shoot regeneration were achieved from explants taken from younger stock plants than mature ones. The results also showed that low concentration of BAP (0.1-0.5 mg/L) were sufficient for shoot induction from explants of both sources. Similar pattern was observed when using combination of BAP and Kinetin. However, explants from mature sources (72 month–old tree) required a longer gestation period up to 15 months to start shoot multiplication whereas for explants from juvenile sources required no gestation period

Keywords: Acacia auriculiformis, micropropagation, juvenile, mature, BAP, Kn

[Download Article - PDF]