E3 Journal of Biotechnology and Pharmaceutical Research

E3 Journal of Biotechnology and Pharmaceutical Research Vol. 3 (4) pp. 74-82, June 2012; © E3 Journals; ISSN 2141-7474

Molecular and biochemical evaluation of anti-proliferative effect of (Cichorium endivia, L.) phenolic extracts on breast cancer cell line: MCF7

Abstract

Medicinal plants are considered to be the most hopeful way for cancer treatment. The Cichorium endivia, L. plant materials were collected from Tanuma, Saudi Arabia. Methanol extraction for the phenolic compounds was carried out and the HPLC analysis showed that, the extract containing four main compounds with different concentrations. The anticancer activity of the root extract was examined on breast cancer cell line MFC7 compared with the anticancer 5 FU (5-fluorouracil). Cytotoxicity of the root extract against the MFC7 line was 401ug/mL but it was 0.67 ug/mL with the 5 FU. The gene expression for the DNA cancer markers; P53, Bcl2, TNF and interleukin IL-4, IL-6 and IL-2 were examined using real time PCR. The expression of the P53 and TNF was high both in cells treated with FU and root extract. Expression of Bcl2 was high in the cell line treated with root extract compared with the FU, yet this expression still was low compared with the control ones. The expression level of IL-2, IL-4 decreased in the examined cell lines treated with both root extract and with 5FU as well. In case of the IL-6 expression was high in cells treated with the extract compared with the treated cells with 5FU and control cell line. Thus, Cichorium endivia, which contains a combination of phenolic compounds, represents an enjoyable means of anticancer especially for breast cancer.

Keywords: Anti-proliferative, Cichorium endivia, Phenolic extracts, Breast Cancer and Cell lineMCF7.