E3 Journal of Biotechnology and Pharmaceutical Research

E3 Journal of Biotechnology and Pharmaceutical Research Vol. 3 (4) pp. 69-73, June 2012; © E3 Journals; ISSN 2141-7474

Genetic relationships among yarrow based on Random Amplified Polymorphic DNA markers

Mohsen Ebrahimi¹^*, Mostafa Farajpour¹, Mina Beigmohamadi², Mehdi Ebrahimi¹
¹ Department of Agronomy and Plant Breeding. Collage of Abouraihan, University of Tehran, Tehran Iran
² Institute of Biotechnology of Urmia University, Urmia, Iran.
^*Corresponding Author E-mail: Mebrahimi@ut.ac.ir/+982923040615, Fax: +982923040615
Accepted 20 April 2012

Abstract

The genetic relationship among 37 Achillea millefolium accessions was studied using Random Amplified Polymorphic DNA (RAPD) markers. Nine RAPD primers were used for the estimation of genetic diversity. All of the 9 primers were polymorphic. Nine RAPD primers generated 118 amplified fragments, most of which were polymorphic. The number of polymorphic bands produced in the 37 yarrow accessions range from 8 to 19. Primer g11 Produced 19 polymorphic bands, while primers e3 produced 8 polymorphic bands. The jaccards similarity indices (J), based on RAPD profiles, were subjected to complete linkage analysis. The highest genetic similarity was observed between Am4 and Am22 (81%), whereas the lowest value was found between Am33 and Am12 (18%). The dendrogram generated revealed five groups. The principle component analysis (PCA) indicated that first three principal component account for more than 62% of the total variation. This study confirms the efficacy of RAPD markers for the identification of plant genotypes. This information should be helpful for breeding and genome mapping programs.

Keywords: Genetic diversity, Achillea milefolium, RAPD, PCA

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