E3 Journal of Biotechnology and Pharmaceutical Research
E3 Journal of Biotechnology and Pharmaceutical Research Vol. 3 (3) pp. 58-61, May 2012; © E3 Journals; ISSN 2141-7474
Mutation of A918C embB gene in ethambutol resistant Mycobacterium tuberculosis in Bandung, Indonesia
Nyi Mekar Saptarini 1 * , Achmad Saifuddin Noer 21 Faculty of Pharmacy, Padjadjaran University, West Java, Indonesia
2 Departement of Chemistry, Institute of Bandung Technology, West Java, Indonesia.
*Corresponding Author E-mail: n_mekars@yahoo.com.
Accepted 20 April 2012
Abstract
Mycobacterium tuberculosis is the agent that causes infectious and contagious tuberculosis disease. The resistance of M. tuberculosis to ethambutol is caused by emb gene mutations. Our research group had isolated and characterized 42 clinical isolates of Multi-Drug-Resistant Tuberculosis using multiplex PCR. Some of these isolates are also resistant to ethambutol, but have not been characterized yet. The purpose of this work is to determine the genotypic basis of the MDR-TB clinical isolate which is resistant to ethambutol (L20). The steps in this work consist of sample selection, amplification of the embB gene, nucleotide sequencing, and in silico analysis. The result showed a substitution mutation, adenine to cytosine at position 918 of the embB gene, which is a first base of codon 306, corresponding to mutation from methionine to leucine.
Keywords: Mycobacterium tuberculosis, ethambutol resistance, embB, A918C substitution
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