Effect of Gentamicin and Amoxicillin on methicillin resistant Staphylococcus aureus (MRSA) against different time and concentrations; Comparative study of the effect of bitter leaf extract and antibiotics (gentamycin and amoxicillin) on bacterial species isolated from wound.

E3 Journal of Biotechnology and Pharmaceutical Research

E3 Journal of Biotechnology and Pharmaceutical Research Vol. 3 (3) pp. 54-57, May 2012; © E3 Journals; ISSN 2141-7474


Antimicrobial activity of the alkaloids and saponin extracts of Anabasis articulata

M. Benziane Maatalah 1 , N. Kambuche Bouzidi 1 * , S. Bellahouel 1 , B. Merah 1 , Z. Fortas 2 , R. Soulimani 3 , S. Saidi 1 , A. Derdour 1
1 Organic synthesis laboratory, Department of Chemistry, Faculty of Sciences, Es-senia University BP 1524 Oran, Algeria.
2 Microbiology laboratory, Department of biology, Faculty of Sciences, Es-senia University BP 1524 Oran, Algeria.
3 Ethnobotanic and Pharmacology (Anxiety, stress oxidant and bioactivity), University P. Verlaine-Metz, Bridoux, Rue du Général Delestraint, 57070 Metz, France.
*Corresponding Author E-mail: kambouche@voila.fr.
Accepted 13 February 2012

Abstract

The crude alkaloids and the saponin extracts of the aerial parts of Anabasis articulata were examined for antibacterial and antifungal activity in vitro using the disc diffusion method. Activity against five bacterial strains (gram positive bacteria and gram negative bacteria) and one fungal strain is discussed. Saponin extract was active against all assayed bacteria (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Klebsiella pneumonia, Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa ATCC 14028,) and a fungal strain (Candida albicans) with minimal inhibitory concentration (MIC) values ranging from 0.5 to 1 mg/ml. Phytochemical screening shows that this plant is particularly rich in alkaloids and saponins which might be responsible for its anticandidal activity.

Keywords: Anabasis articulata, Antibacterial activity, Antifungal activity, disc diffusion method, Saponins, alkaloids.

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